Product photo
Product details
Name: Burkholderia phytofirmans Biotin synthase (bioB) -E. coli
Size: 1000ug
Catalog no.: GEN1183082.E.coli
Order on Gentaur.comGene name
bioB
Expression system
E. coli
Conjugation
Biotinylated
Form
Lyophilized protein
Product category
Recombinant Proteins
Other gene names
Bphyt_0444; bioB; Bphyt_0444
Purity
Greater than 90% (determined by SDS-PAGE)
Available also expressed in:
E Coli ; Yeast ; Baculovirus ; Mammalian Cell
Alternative names
biotin synthase; Biotin synthase; biotin synthase;
Long name
Recombinant Burkholderia phytofirmans Biotin synthase (bioB)
Applications
This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc.
Storage
This protein can be stored at -20 degrees Celsius. For extended periods of time it is recommended to keep the protein frozen at -40 or -80 degrees Celsius. Avoid cycles of freezing and thawing as they might denaturate the polypeptide chains.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
General description
Biotin synthase(bioB) is a recombinant protein expressed in E. coli. The protein can be with or without a His-Tag or other tag in accordance to customer's request. All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility.