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Product details

Name: Phytochrome A, photoreceptor PhyA protein

Size: 200 µg

Catalog no.: AS07 220

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Protein number

P14712

Raised in

Rabbit

Molecular weight (expected | аpparent)

124 kDa

TAIR number

AT1G09570

Clonality

Polyclonal

Clone

Polyclonal

Recommended dilutions for use

1: 1000 (WB)

Verified applications

western blot (WB)

Purification

Affinity purified serum

Available ordering format

Lyophilized in PBS pH 7.4

Verified reactivity

Arabidopsis thaliana, Nicotiana benthamiana

How to reconstitute

For reconstitution add 200 µl of sterile water.

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

careful sample collection is adviced to assure the best results with this antibody

References

Paik et al. (2012). Phytochrome regulates translation of mRNA in the cytosol. PNAS 109 (4): 1335-1340.

Notes

in vivo pull down assay for PhyA and western blot analysis of eluted proteins is described in this paper.

Possible reactivity

Brassica rapa, Lathyrus sativus, Fragaria ananassa, Glycine max, Lotus corniculatus, Medicago truncatula, Nicotiana benthamiana (PhyA1), Nicotiana tabacum, Pisum sativum, Populus balsamifera, Ricinus communis

Connected products

AS06 198 | anti-FT/TSF | flowering locus T and twin sister of FTAS16 3158 | anti-PhyB | phytochrome B proteinAS16 3147 | anti-SPA1 | supressor of PhyA-105AS12 2112 | anti-PIF5 | phytochrome interacting factor 5

Scientific context

Phytochrome A (PhyA) is the primary photoreceptor mediating various responses to far-red (FR) light in plants. Alternative protein names: FAR RED ELONGATED HYPOCOTYL 2, FAR RED ELONGATED 1, ELONGATED HYPOCOTYL 8.

Immunogen

KLH-conjugated synthetic peptide derived from conserved plant PhyA protein sequences including Arabidopsis thaliana UniProt:P14712, TAIR:At1g59070; peptide sequence is not present in other plant phytochrome forms (B-E)

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

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